This should work for your case;
assert not my_var.called, 'method should not have been called'
Sample;
>>> mock=Mock()
>>> mock.a()
<Mock name="mock.a()" id='4349129872'>
>>> assert not mock.b.called, 'b was called and should not have been'
>>> assert not mock.a.called, 'a was called and should not have been'
Traceback (most recent call last):
File "<stdin>", line 1, in <module>
AssertionError: a was called and should not have been