IIS 8.0 ASP.NET and Error 500.19

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ASP.Net applications come pre-wired with a handlers section in the web.config.

By default, this is set to readonly within feature delegation within IIS.

Take a look in IIS Manager, under the Server name → Feature delegation.
If Handler Mappings is set to readonly and your site web.configs have a handlers section, flip the value to read/write and see what happens.

Worked for me when moving to Windows 8 / IIS 8 / ASP.Net MVC 4.

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