WSGI is the Python standard for web server interfaces. If you want to create your own framework or operate without a framework, you should look into that. Specifically I have found Ian Bicking’s DIY Framework article helpful.
As an aside, I tend to think frameworks are useful and personally use Django, like the way Pylons works, and have used Bottle in the past for prototyping—you may want to look at Bottle if you want a stay-out-of-your-way microframework.