How do I run a single test with Nose in Pylons

nosetests appname.tests.functional.test_controller should work, where the file is named test_controller.py. To run a specific test class and method use a path of the form module.path:ClassNameInFile.method_name, that is, with a colon separating the module/file path and the objects within the file. module.path is the relative path to the file (e.g. tests/my_tests.py:ClassNameInFile.method_name).